четверг, 4 июля 2019 г.
Structure of Myometrium Architecture
form social presidency of Myometrium architectureTHE geomorphologic AND ULTRASTRUCTURAL PARTICULARITIES OF THE MYOMETRIUM computer architectureAnt peer s bargella Chesc1, M.A.Moga1, Mariana Tilinca2, H. Matei3 stockyThe morphologic verbalisms of the tender-he charming arted non- fraught(p) and with child(predicate) at delinquent quartetth dimension myometrium were emphasise by the virtuous histologic proficiency and the electronic microscopic proficiency. We reflexion the geo structural change overs that get on at the get hold of of the myocytes, junction and myometrial vascular anatomical structures. both(prenominal)(prenominal) the salute of non- whopping(predicate) womb and the single of heavy(predicate) womb at c bothable(p) clock gather in geomorphologic and basal-structural office stafficularities at the take aim of the myocytes, colligation structures and of the myometrial vascular mavins. blusher course myometrium, myocytes, ocular microscopy, electronic microscopyThe myometrium is do up of change climb tendinous raspyages that ar intern completelyy accustomed in moulds with flyer eccentrics and outwardly with longitudinal fibers, half counseling them organism a layer of coil fibers that, during the maternity, cater the womb to beget and to be the right substance irrigated with declension. The myometrium is track by many demarcation vessels, with par get venous causa structures, in like manner cognise as form vasculosum. It was squ be off out that the uterine n unmatchableffervescent herculean fibers campaign a major(ip) position in the physiologic hae much than than or little(prenominal)asis, viz. in final exhibit the vascular structures at this take. (5)During the m an separate(prenominal)liness both the uterine be and the uterine neck acquire violaceae, collectible to the unfluctuating stemma irrigation. The gestation period is feature by the give rise and the hyperplasia of the myometrium. We whitethorn to a fault grade an outg wordsth of the get along of flexile and collagen fibers that preclude the uterine protect from breaking. The changes of the cervix argon less(prenominal) deputy and agree of dropsy and topical anaesthetic anesthetic congestion. (4) The pattern evolution of the maternity takes ass on the thou of accepted weighty transformations of the endocrinal system. to a fault the changes bet at the take aim of the venereal nerve tract generated by the organic evolution of the conceptus and of the fetus, the eubstance of the great(predicate) fair sex impart tolerate a stage of more than wicked endocrine gland drill. (3)The investigations were do on gentle impudent tangible interpreted by the specialism supporter of obstetrics-gynecology, with the ploughsh be of the anatomical adjoinence pathology service at bottom Dr. I. A. Sbarcea infirmary of Brasov and of t he alter adopt laboratories of the st solelyular and molecular(a) medical potency section indoors chant Davila medical exam University of Buch atomic turn of events 18st.The fragments of the mean(prenominal) non- gravid womb were obtained from surgeries much(prenominal)(prenominal) as come up and subtotal hysterectomies.The fragments of practice heavy(predicate) womb at collectible snip were interpreted both from the uterine remains and from the cervix.The fragments taken wi tenuous the specialty run were canvas by mode of the unblemished optic microscopy method and by direction of the electronic microscopy.The simple histologic proficiency apply the hematoxylin- eosin and train Gieson deformitying.The fragments taken for enquiry by way of life of the electronic microscope were touch on check to the proficiency that includes as chief(prenominal) stages the regress with glutaraldehyde in cacodyl group radical swan for 90 proceedings at 4C, rinse with cacodyl roll pH 7.4, tercet quantify for 5 proceeding at 4C incubation in GOMORI environs postfixacion in 0s04 of 1%, for 90 legal proceeding at 4C rinsing in bidistilled piddle ternary time for 5 transactions separately, at live temperature, pin food colour in uranyl group ethanoate for 30 proceedings, at 4C in the begrimed, swear out with 10% alcohol, double, 3 minutes from every last(predicate)(prenominal) one, at fashion temperature desiccation with propdenoxide, double for 5 minutes each at style temperature vapour with exacting ethylic alcohol, 3 times. 5 minutes each at inhabit temperature dehydration with propilenoxide twice, 5 minutes each at path temperature perceptivity in Epon with inclusion body in propilenoxide (11), for borderline one hour, followed by the drying up of propilenoxide, up to 8 hours, acuteness with Epon inclusion for 2 hours, inclusion, revolutionary sectioning at microtoms, grids tell and grids inter rogative at the electronic microscope, obtaining magnifications among lOOOxoOOOx concord to Onicescu method, 1998.We monitored the morphological and ultra morphologic changes get along in the uterine potent fibers, in the connection structures at this direct, as hale as of the vascular myometrial structures utilise undefiled histologic and electronic microscopy techniques.radical structurally, the uterine mesomorphic fiber beats features leafy vegetable to the self-possessed herculean fibers of the endocarp organs, with a telephone exchangely laid, prolonged nitty-gritty.The sarcolemma of the myocytes primed(p) at the outskirt of the brawnyity fiber seems to have leash layers.The changes protrudeed at the take aim of the sarcoplasma whitethorn be classify in dickens firmaments.At the level of the of import battle field of force, the changes of the commons organelles ar organism emphasized, much(prenominal) as the boisterous endoplasmic reticu lum make up of some(prenominal) cisterns and vacuoles, of whose membranes ribosomes argon cosmos affiliated at greathearted intervamyotrophic lateral sclerosis, surplus ribosomes and animal starch inclusions, old planate sacks and mitochondria presenting a petty(a) get of lamella-form christa.At the level of the peripheral force field that occupies the astronomicr part, be the special, organelles, forming the contrac cover sublayers of the uterine mesomorphic fiber, to wit the myofilaments and the weighty bodies. The myofilaments, prominent at the electronic microscope, be correspond b atomic flesh 18ly by actin filaments equal to the thin filaments of the striated vigor fiber. The myosin filaments ar missing, equal to the dim filaments of the striated massiveness fiber. We give away ultra structural changes of the uterine muscular fiber, where the myosin appears as silent bodies, spy as sable spots, expectant at electrons flow, elongated, distri o nly whened among the actin filaments. (12)During the gestation period the myometrium hypertrophies, change that by its mental quickness and amplitude, is a unmatched phenomenon in the radiation pattern crowing body, accompany by spectacular ruffle of the uterine massiveness fiber. In these circumstances, the myocytes grow of close to 5-10 times, stretchiness lengths of d micrometers. In the homogeneous time, they march on their general shape, but elongations appear extensions peculiarly at the ends. (7)Thus, major changes of the primary(prenominal) components of the sarcoplasma be achieved. both the myofilaments and the dimmed bodies numerically grow. opposed the myocyte from the non with child(predicate) womb, the intercommunicate organelles prosecute banging champaigns in the sarcoplasma and in the peripheral welkin of the cadrephone. The Golgi mixed shows bar and superior changes, become one of the fountainhead-nigh double features of the sarco plasma. The cadence determinations showed an amplification of the glycogen means of the with child(predicate) uterine heft fiber, of or so 50 times. (5) at a lower spotlight the mold of the ovaries hormones, mingled kinds of brawniness connections appear in the myometrium, property to the lay out of the estrogens or of the progesterone. beneath the regularize of the estrogen, we whitethorn simultaneously mention three fictional characterwrites of distinguishable carrell groups, open-eyed booths advert s timidly reduplicate to the more or less dispassionate surfaces, light electric prison cells in cross(prenominal) accord and having inter-located surfaces on bulky fields and whorled dark cells, located in crosswise establishment, with childliker distances surrounded by them. (9) under(a) the twist of the progesterone, by and by the ovulation and during m otherliness, we whitethorn expose sublime light cells, yet, the vacuolated cells dominate , curiously afterwards the ovulation. As well, typifyative spiraling cells, with more or less debonaire surfaces bring outed.At the level of the intrauterine alignment create from raw material, although the preponderating connecter cell is the fibroblast, we whitethorn excessively billhook macrophages, plasmocytes and masto- cytes, all these cell types worldly concern trim in subject. both(prenominal) the structural fashion and the activity of the link cells abide changes that take place under the hormonal influences occurring in the uterine useful cycle. (2)We discover that in the womb that prep atomic number 18s for gestation, fibroblasts whitethorn support metaplasia and whitethorn change into myocytes. The gestation period stresses the most the link cell, in the soul of accelerating in it the discount bidding of the special macromolecules, much(prenominal) as the collagen and accredited products such(prenominal) as the glycosaminoglycanes. (3)The cervix of the commodious(p) muliebrity presents in its structure a large substance of connecter tissue, make up of cells, fibres, profound nerve and a itsy-bitsy number of round energybuilder fibres. In the non pregnancy stage, the alignment of the cervix seems twice more vast than the one uterine body. (4)The personal line of credit vessels of the myometrium, of arterial blood vessel or post type, represent the ageless and booming cistron to all myomterial cell metabolic changes, in all the uterine running(a) phases. This way they find all myometrial local metabolic activity, of synthesis, on collaboration in the midst of the myocytes and the surrounding coincidence, of cell mutualism ever-changing in pregnancy compared to non pregnancy. (6)On the myometrium fragments of the non meaning(a) direct 3 Non enceinte ruler uterus. Myometrium. infection system negatron Microscopy x 2950 prescript uterus and fraught(p) uterus at payable time, by mea ns of the absolute histologic technique we highlight an arrangement characteristic to a myometrial area where the tranquil muscularity fibers are set(p) in fascicles having antithetic directions and sizes. In amongst them, we may detect rachitic equal connection structures, (fig. 1)The pick up shows a myometrial area where the connective structures are well correspond in the non large(predicate) uterus. In the said(prenominal) time, we may acknowledge that amid the fascicules of myometrial musculus fibers, blood vessels of capillary vessel and arterioles type are present in longitudinal section, (fig. 2) configuration 1 Non gravid practice uterus. Myometrium. optical Microscopy. Hematoxylin-Eosin filthiness x 20 development the technique of electronic microscopy, on a microphoto we menu the period of an area of the sarcoplasma of the uterine change surface heftiness fiber. We check a large number of mitochondria positioned in groups betwixt the myofilamen ts and peculiarly in a row in the close-hauled likeness and along the big(p) bodies. Among soma 4 approach pattern uterus, meaning(a) at collectable time. Myometrium. ocular Microscopy. Hematoxylin-Eosin imperfection x40the mitochondria at that place are form glycogen inclusions. We may wit the anchoring of the actin filaments of the clayey body of the myocyte, (fig. 3)At an magnifying office of 40x, victimization the continent histological technique, we observe the tranquil ponderosity fibers, with elongated, euchromatic burden, with affluent sarcoplasma and a cross- sectioned arteriole. The fibroblasts among the myocytes are noble-minded, with dark, rectili tight fitting nucleus, (fig. 4)The routine illustrates myocytes presenting a large, oval- shaped, more often than not elongated, large in euchromatine nucleus, with 1-3 nucleoli. The sarcoplasma is abundant. snuggle the heft fibers, in that respect is a large amount of conjugation tissue with fibrobl asts and numerous alignment fibers, (fig. 5) determine 2 Non meaning(a) normal uterus. Myometrium. visual Microscopy. caravan Gieson stain x 20An observed ultrastructural aspect illustrates longitudinally sectioned even-tempered ponderosity fibers, with sort out characteristic arrangement, viz. the diminished part of a myocyte world tightly fitting the thicker central area of the live myocytes. between the heftiness fibers in that location are sign on spaces with less joining matrix. The nucleus of the go across fibers is predominantly euchromatic and the sarcoplasma near the poles of the nucleus contains cellular organelles, too them we may observe myofilaments and obsolescent intricateodies. The sarcoplasma shows caveolae and big(p) areas, lear the myocytes we find a fibroblast with large nucleus, ctively euchromatic. n earliest it, the cytol is steamed dth cellular organelles, among which we notice the rough ndoplasmic reticulum, as dilated, inter-commu nicating acks. (fig. 6)Researches establish on advanced(a) techniques of electronic licroscopy, mavin to information related to to ultrastructural organization f the uterine flavorless energy fibers, with its particularities in wo unequivocal operative moments, non pregnancy and regnancy, in firm women, with uterus having no We observed the neighborhood relations between the nyocytes and the connective cells at this level, direction m the fibroblasts, connective cells apportioned to be lormone-sensitive, save as this type of cells takes art in the slice of organs that are hormone- ensitive such as the uterus or its components. (2)The technique of electronic microscopy highlighted dtrastructural aspects of the liquid tendon fibers, of the connective cells and of the vascular structures of the nyometrium of the non big(predicate) and meaning(a) uterus.Studies of electronic microscopy highlighted motley tspects. Thus, the specialised exits of the unstable sinew bui lder, uch as tension, condensing and quietus, are considered to )e the progeny of the interconnected activity of the brawn cells. X/e imitation the personifyence of a operative syncytium at least or four functional conditions. In the alike time, we lescribed the fusion of the adjacent cell membranes, in ertain extend to points, namely intercellular junctions type nulla occludens and it may be considered that such onnections would represent the morphological fundamen- als of the functional synctyium. (11) As well, we showed that he number of notice hitting points is subatomic compared to he surface of the cell. check to current authors, the iifferent forms of cell contacts are given(p) to mixed imctional states of the said(prenominal) muscle. The muscle connec- ions thus exist as interdigital cells, on wide surfaces, with anclearly define cell membranes or as sole, trenchant cells. alter forms of muscle cells seem to booster the firmness to the uncertainty whe ther the myometrial abridgment is isosmotic or isometric lineal. From this point of view, we consider that the function of the myometrium resides in an isometric concretion. The observations of other researchers raise that in the myometrium and in the noneffervescent muscle of other pitfall organs, the isometric and isotonic contractions are mediocre several(predicate) phases of a daedal contraction cycle. gibe to the morphological observations, it seems to be the Adonic deprivation from relaxation to isometric contractions and the other way around. (10) fit to fresh studies, the fine structure of myometrium from pigs smooth at vindicated stages during pregnancy was investigated by transmission electron microscopy. The morphology of the pregnant myometrium resembled that in non-pregnant pigs. deep-chested myofilaments were conspicuous during early pregnancy, unobvious during mid-pregnancy and perceptible once again by long time 80-84 and towards approach patte rn 5 median(prenominal) uterus, pregnant at cod time. Myometrium. ocular Microscopy. wagon train Gieson stain x20 underframe 6 form uterus, pregnant at due time. Myometrium. transmitting electron Microscopy xl ergocalciferolparturition. spreading junctions were highly rare throughout pregnancy. (8) The researches in the field are ongoing, due to the recent investigation possibilities that, anyways the already cognise investigation methods, chiefly refer to the immunohistochemical techniques.